The Journal of American Science
(J Am Sci)
ISSN 1545-1003 (print); ISSN 2375-7264 (online),
doi prefix:
10.7537, Monthly
Volume 12, Issue 5, Cumulated
No. 99, May 25, 2016
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CONTENTS
No.
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Titles / Authors
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Full
Text
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No.
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1
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Determinants and Consequences of Perceived Organizational
Support (P.O.S) on Organizational Commitment (O.C): A Literature
Review Approach
Dr. Khaled N. Alshuwairekh
Dean college of Business - Assistant
Professor Dar Al Uloom University, Riyadh, Kingdom of Saudi
Arabia.
khalshuw@yahoo.com
Abstract:
In view of rapidly changing business environment, companies must
devote greater effort to enhance their capabilities and it must
not be forgotten that the success and competing power of the
organization depend on committed, highly motivated, satisfied
and innovative human resources. While discussion of perceived
organizational support has been prevalent in the popular
literature for many years, However this concept needs to be an
ongoing study by researchers due to rapidly changing business
environment.
The main purpose of this study is to examine the effects of
perceived organizational support on organizational commitment.
In this research, organizational commitment was examined in
terms of affective commitment, normative commitment and
continuous commitment.
This study is descriptive based on the collection and analysis
of results of studies, reports, periodicals and books related to
the topic of study in order to investigate the relationships
between Perceived Organizational and organizational commitment.
The study is driven theoretically by the social exchange theory,
the concept of perceived organizational support, which is the
commitment of the organization towards the employee, the norm of
reciprocity as well as the other relevant literature in the
human resource management and organizational behaviour research.
Based on descriptive study, the researcher has found a positive
relationship between Perceived Organizational and organizational
commitment, however, the researcher has develop a new managerial
checklist to improve using perceived organizational support
within the organization.
[Khaled N. Alshuwairekh.
Determinants and Consequences of Perceived Organizational
Support (P.O.S) on Organizational Commitment (O.C): A Literature
Review Approach.
J Am Sci
2016;12(5):1-9].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
1.
doi:10.7537/marsjas120516.01.
Keywords:
perceived Organizational Support, Organizational Commitment,
affective commitment, normative commitment, continuous
commitment, Job Conditions,rewards, fairness and supervisor
support. |
Full Text |
1
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2
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Quinoline-based small molecules as effective protein kinases
inhibitors
(Review)
Rasha M.
Aly 1*, Rabah A. T. Serya2, Amira M. El-Motwally
1, Ghada H. Al-Ansary2, Dalal A. Abou El
Ella2.
1National
Organization for Drug Control and Research, Cairo, Egypt.
2Pharmaceutical
Chemistry Department, Faculty of
Pharmacy, Ain Shams University, Abbassia, Cairo, Egypt.
rashamohamed83@hotmail.com
Abstract:
Cancer,
malignant tumor,
is a group of diseases characterized by abnormal and out-of-controlcell
growth with the potential to metastasize to other sites in the
body. Protein kinases are a class of molecular drug targets
forcancer treatment. Identification of the protein kinases key
roles in development of cancer leads to the discovery of
different classes of protein kinase inhibitors with enhanced
selectivity and efficacy and reduced toxicity. In this review,
we focused on the quinoline derivatives as an important class of
compounds having potent inhibition effect on the activities of
different protein kinases.
[Rasha M. Aly, Rabah A. T. Serya.
Amira M. El-Motwally, Ghada H. Al-Ansary,
Dalal A. Abou El Ella.
Quinoline-based small
molecules as effective protein kinases inhibitors.
J Am Sci
2016;12(5):10-32].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
2.
doi:10.7537/marsjas120516.02.
Keywords:Quinoline,
kinase inhibitor, anticancer. |
Full Text |
2
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3
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Biologically Active
Metabolites from Penicillium
sp., An Endophyte Isolated from Glaucium arabicum
Ahmed Elbermawi1, Amal
Sallam1, Ahmed Ashour1, Weaam Ebrahim1,2,
M.-F. Lahloub1 and Hassan-Elrady A. Saad1
1Department
of Pharmacognosy Faculty of Pharmacy, Mansoura University,
Mansoura, 35516 Egypt.
2Institute
of Pharmaceutical Biology and Biotechnology, Heinrich-Heine
University, 40225 Düsseldorf, Germany.
amal14sallam@yahoo.com
Abstract:
A heterodimericxanthone,
secalonic acid G (1), a diketopiperazine alkaloid,
3-[(1H-indol-3-yl)
methyl]-6-benzylpiperazine-2,5-dione
(2)
and ergosterol (3)
were isolated from the solid rice culture
of a Penicillium sp., an endophyte isolated for
the first time from both the stems of Glaucium arabicum
Fres. and the Papaveraceae. The structures were elucidated on
the basis of 1D and 2D NMR data.
Both compounds 1 and 2
were evaluated for their antioxidant, antibacterial, anti-melanogenesis,
cytotoxic, collagen production promoting and hyaluronic acid
production promoting activities.
Secalonic acid G (1)
demonstrated a strong and
selective antibacterial activity against S. aureus, a
potent cytotoxic activity against both B16 melanoma and RBL-2H3
cell lines and a significant antioxidant activity. Both
compounds showed an inhibitory effect on the production of both
hyaluronic acid and collagen but this effect is accompanied by a
significant and important increase in the cell viability
suggesting their effective use for wound healing.
[Elbermawi A, Sallam A, Ashour A,
Ebrahim W, Lahloub M.-F, Saad H-EA.
Biologically Active Metabolites
from Penicillium sp., An
Endophyte Isolated from Glaucium arabicum.
J Am Sci
2016;12(5):33-41].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
3.
doi:10.7537/marsjas120516.03.
Keywords:
Glaucium arabicum,
Papaveraceae, endophytes, Penicillium sp., secalonic acid
G, diketopiperazine alkaloid, antibacterial, anti-melanogenesis,
cytotoxicity, antioxidant. |
Full Text |
3
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4
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Anabolic Steroid and
nutritional supplements
effect on kidney
Sameh Abouzeid, Osama Mosbah,
Mohamad Hegazy
Theodor Bilharz Research
Institute Nephrology Department Cairo, Egypt
sameh.kidney@gmail.com
Abstract: Background:
Frequent use of anabolic steroids and dietary supplements for
the purpose of the muscle mass is common among body builders.
Recent evidence indicates that anabolic steroids are directly
toxic to glomeruli and that segmental sclerosis is the result of
podocyte loss mediated by apoptosis through a podocyte androgen
receptor.
The high-protein intake has been of concern to nephrologists
because it increases glomerular filtration rates and is
experimentally associated with glomerular hyperfiltration and
FSGS. The aim of the
present study was to asses and compare the effect of anabolic
steroids and other nutritional supplements on kidney outcomes.
Methods:
Twenty male body builders
volunteered to participate in this study, age between (20 and 35
years), Volunteers were divided into two groups. Group 1
(Steroid group) consisted of ten male volunteers use anabolic
steroids (more than two years and less than 5 years) with
concomitant use of whey protein, creatine monohydrate and
Branched chain amino acids intermittently for more than 2 years.
Group 2 (Non-Steroid group), included ten male volunteers use
whey protein, creatine monohydrate and Branched chain amino
acids intermittently for more than two years.
Results:
There is significant increase in the amount of protein in 24
hours of collected urine (mg/24 hrs.) in steroid group (group 1)
when we compare its level to non steroid group (group 2) (24
hrs. urine ptn: 859.50 ± 805.72 vs. 128.50 ± 48.25, p<0.001).
Conclusion:
Long use of AASs could produce renal effects as variable
elevations in serum creatinine and development of substantial
proteinuria.
[Sameh Abouzeid, Osama Mosbah,
Mohamad Hegazy. Anabolic Steroid and nutritional supplements
effect on kidney.
J Am Sci
2016;12(5):42-45].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
4. doi:10.7537/marsjas120516.04.
Keywords:
anabolic steroids, nutritional supplements, FSGS |
Full Text |
4
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5
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Prediction of Weft Breaks in
Air Jet Weaving Machine by
Artificial Neural Network
Shaimaa
Youssef El-Tarfawy
Textile
Engineering Department, Faculty of Engineering, Alexandria
University, Egypt
Abstract:
The most important factors affecting the air jet weaving
machine’s efficiency are warp and weft breaks which are strongly
connected to the yarn and machine parameters. The objective of
this paper is to predict the number of weft breaks per million
meters using back propagation algorithm in an artificial neural
network system. Two models are used: first utilizing twelve
parameters for the input layer and second applies only the five
most correlated yarn parameters.
The developed algorithm can predict the number of weft breaks
per million meters. The results gave satisfactory coefficient of
correlations (0.955) between the actual and predicted number of
weft breaks.
[Shaimaa Youssef El-Tarfawy.
Prediction of Weft
Breaks in Air Jet Weaving
Machine by Artificial Neural Network. J Am Sci
2016;12(5):46-51].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
5.
doi:10.7537/marsjas120516.05.
Keywords:
Weft breaks; Yarn properties; Artificial Neural Network; Mean
square error; Prediction model |
Full Text |
5
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6
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Failure Behavior of Polyester/
Lycra Single Jersey Knitted Fabric
Shaimaa
Youssef El-Tarfawy
Textile
Engineering Department, Faculty of Engineering, Alexandria
University, Egypt
Abstract:
The bursting strength property is one of the most important
mechanical properties of knitted fabrics and varies according to
different parameters. This study investigated the failure
behavior of four different samples with different percentage of
polyester/ lycra single jersey knitted fabric. Tensile and
bursting strength were measured for the samples. The most
important conclusion is that adding Lycra loaded the loop with
un-balance forces and uneven
distribution of stresses in the wale and course directions.
Tensile, bursting strength and Young’s modulus gave high
coefficient of correlation with lycra percentage.
[Shaimaa Youssef El-Tarfawy.
Failure Behavior of Polyester/ Lycra Single Jersey Knitted
Fabric. J Am Sci
2016;12(5):52-58].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org. 6.
doi:10.7537/marsjas120516.06.
Keywords:
Lycra, single Jersey, tensile strength, bursting strength |
Full Text |
6
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7
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Significance of TGF-β1 and
Catheps in E expression in gastric adenocarcinoma and
precancerouslesions
Hanaa Abd Elghany Ahmed Atwa and
Shimaa Ahmed Ahmed Arafa
Department of Pathology, Faculty
of Medicine, Zagazig University, Egypt.
aragab818@gmail.com
Abstract: Background and aim:
40
cases with gastric lesions comprise the material of this study.
These include 40 paraffin blocks selected from gastric specimens
(30 cases were obtained
by endoscopy and 10 cases were surgical specimens)
received at the pathology department,
Faculty of Medicine, Zagazig University in the period from
September 2013 to September 2014. The aim of this study
to examine the expression of transformed growth factor beta 1 (TGF-β)
and Cathepsin E (CTSE) in
gastric cancer and gastric precancerous lesions and to correlate
between these markers and clinicopathological parameters.
Methods: Immunohistochemical analysis of TGF beta and
Cathepsin E were performed using 40 cases for chronic
superficial gastritis, chronic atrophic gastritis, chronic
atrophic gastritis with intestinal metaplasia, gastric dysplasia
and gastric cancer.
Results:
In cases of chronic gastritis, we observed only TGF-β1
reactivity in the cytoplasm of inflammatory cells mainly plasma
cells. In intestinal metaplasia and dysplastic lesions, the
reactivity to TGF-β1 was more intense than in normal mucosa,
especially in the cytoplasm of the Goblet cells. TGF-β1 was
expressed in 88.8% of incomplete intestinal metaplasia. TGF-β1
expression was detected in intestinal type of gastric
adenocarcinomas in 100% of well differentiated adenocarcinoma,
50 % of moderately differentiated adenocarcinoma and 66.6% of
poorly differentiated adenocarcinoma. TGF-β1 was expressed in
50% of papillary carcinoma and mucinous carcinoma. TGF-β1was
expressed in 100% of signet ring carcinoma.
In
chronic gastritis without atrophy or intestinal metaplasia,
expression of cathepsin E is clearly observed in the fundic and
pyloric glands of stomach. CTSE immunestaining was detected in
100% of type II (incomplete intestinal metaplasia) it is
negative in type I IM (complete intestinal metaplasia). CTSE was
expressed in 57.14%
of gastric cancer. More undifferentiated gastric tumors tend to
increase expression of CTSE in tumor lesions (poorly
differentiated adenocarcinoma> moderately differentiated
adenocarcinoma> well differentiated adenocarcinoma). Cathepsin E
was negative in mucinous and papillary carcinoma (100%)
negative expression. Cathepsin E was
positively expressed in 100% of signet ring carcinoma.
Conclusion:
The
alterations of expression of TGF
and cathepsin E can determine the histological variants
of gastric cancer (GC).
Cathepsin E could be a marker for signet ring carcinoma
Understanding the expression pattern of TGF beta and Cathepsin E
in gastric cancer allows them to be used as future therapeutic
targets.
[Hanaa
Abd Elghany Ahmed Atwa and Shimaa Ahmed Ahmed Arafa.
Significance of TGF-β1 and Cathepsin E expression in gastric
adenocarcinoma and precancerouslesions.
J Am Sci
2016;12(5):59-67].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
7. doi:10.7537/marsjas120516.07.
Key words:
cancer stomach, TGF-β1,cathepsin E,
Immunohistochemistry. |
Full Text |
7
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8
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The impact of
E-commerce on Business Strategy:
A Literature Review Approach
Amir Abou Elnaga1 and Frahan Hassan Al Shammari2
1Assistant
Professor, Director of Graduate Studies, College of Business
Administration, Dar Al Uloom University, Riyadh, Kingdom of
Saudi Arabia.
2
Master of Business Administration, College of Business
Administration, Dar Al Uloom University, Riyadh, Kingdom of
Saudi Arabia.
amirzaki78@hotmail.com
Abstract:
E-commerce is a new business model in the world as it is
prompted a major change in the mode of production and services
of the whole world. That with the rapid development of Internet
technology, e-commerce has become a key means of modern
enterprise competition. This study is descriptive based on the
collection and analysis of results of studies, reports,
periodicals and books related to the topic of study in order to
investigate the relationships between E-commerce on Business
Strategy. The main purpose of this study is to describes the
influence of e-commerce on the business strategy in order to
understand electronic commerce, models and types
Business-to-Consumer (B2C), Business-to-business (B2B),
Consumer-to-consumer (C2C), Intra-organizational and describing
the difference between e-commerce
and e-business in order to determine the impact of E-commerce on
Business Strategy also E-commerce characteristics from worldwide
perspective like United State of America, Korea, Kenai has been
reviewed. Based on descriptive study, the researchers has found
a positive relationship between E-commerce and business Strategy
through characteristics of E-commerce systems and benefits of
adopting e-commerce. also E-commerce does not change the
business nature but it is a complementary tool that supplements
the overall strategy and enhances the business performance. An
organization capability to manage technology-enabled change is
the core of successfully managing e-business. The step of change
and the opportunities for new communications approaches make
e-business and e-commerce an exciting area of business to be
involved in.
[Amir
Abou Elnaga and Frahan Hassan Al Shammari.
The impact of
E-commerce on Business Strategy:
A Literature Review
Approach.
J Am Sci
2016;12(5):68-79].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
8.
doi:10.7537/marsjas120516.08.
Keywords:
E-commerce, Business Strategy, e-business, Business-to-consumer
(B2C), Business-to-business (B2B), Consumer-to-consumer (C2C),
Intra-organizational, marketing mix |
Full Text |
8
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9
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Activity of G6HD, ME, GPI and
protease enzyme in Cryptosporidium oocyts isolated from
Immunocompetent and Immunocompromised patients.
Dr. Nada M. Al-Bashir1,
Dr. Huda T. Al-Marsomy2,
Dr. Jabbar Salman Hassan3,
Ahlam Ismail 4
1, 2
and 3 Ph.D. Medical Microbiology/ College of
medicine, Al-Nahrain University
4
M. Sc. Medical Microbiology
Al-Kadhimyia Teaching Hospital
jabbarsalman30@yahoo.com
Abstract:
Cryptosporidiosis is a clinical disease, usually presents as a
gastro-enteritis-like syndrome, caused by infection with
protozoan parasites of the Apicomplexan genus Cryptosporidium.
Disease ranges in seriousness from mild to severe and signs and
symptoms depend on the site of infection and nutritional and
immune status of the host.
This study was conducted to evaluate the activity of enzyme
Glucose Phosphate isomerase (GPI), Malic Enzyme (ME),
Glucose -6-Phosphate
Dehdrogenase (G6PD) and Protease Enzyme in oocytes of
Cryptosporidium species and if their significant difference
between activity of oocytes isolation from immunocompetent
patients or immunocompromised patients. Six hundred patients
with an age range from 12 day to 18 years suffering from
diarrhea. Modified Acid – fast stain (modified Ziehle-Neelsen)
will be used in diagnosis of Cryptosporidium oocytes.
Sixty six samples (11%)
out of 600 patients were positive for Cryptosporidium oocytes by
using modified Ziehle-Neelsen stain, regarding Enzyme activity
results show highly activity of all enzymes included in this
study with no difference in enzyme activity between
Immunocompetent and immunocompromised patients. In this study we
conclude that GPI, ME, G6PDH and Protease enzymes were found in
Cryptosporidium Oocytes with highly activity, there was no
significant difference between oocytes activity isolated from
immunocompetent and immunocompromised patients.
[Nada M. Al-Bashir, Huda T.
Al-Marsomy,
Jabbar Salman Hassan, Ahlam
Ismail. Activity of G6HD, ME, GPI and protease enzyme in
Cryptosporidium oocyts isolated from Immunocompetent and
Immunocompromised patients.
J Am Sci
2016;12(5):80-84].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
9.
doi:10.7537/marsjas120516.09.
Keywords:
Cryptosporidium, G6HD, ME, GPI, Protease |
Full Text |
9
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10
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Exploring the
relationship between brand image and consumer purchasing
decision: A Theoretical Framework
Huda Almutlaq
MBA, College of Business
Administration, Dar Al Uloom University, Riyadh, Kingdom of
Saudi Arabia
Huda.mutlaq@msn.com
Abstract:
The purpose of this study is to identify the importance of brand
image in business matters, besides, exploring the relationship
between brand image and
consumer purchasing decision. The
Findings of this research showed that brand image has strong
positive influence & significant relationship with Consumer
buying behavior. Thus, companies should take all necessary
procedures to develop their brand images and maintain their
position in the competitive business. This study is descriptive
based on the collection and analysis of results of studies,
reports, periodicals and books related to the topic of study in
order to exploring relationship between brand image and
consumer purchasing decision also reviewed the model of
consumers decision making process.
Based on descriptive study, the researcher has
develop some main guideline to develop positive relationship
between brand image &consumers
purchasing decision.
[Huda Almutlaq. Exploring the
relationship between brand image and consumer purchasing
decision A Theoretical Framework.
J Am Sci
2016;12(5):85-92].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
10.
doi:10.7537/marsjas120516.10.
Keywords:
brand image, consumer-purchasing decision |
Full Text |
10
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11
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The Impact of Rehabilitation through Urban Regeneration on
cultural dimensions for the old markets: the case of Souk Al
Sokar, Amman, Jordan
Jawdat Goussous1, Maryam Nofal2
1.
Department of Architectural Engineering, Faculty of Engineering
and Technology, the University of Jordan, Amman-Jordan,
Assistant Professor,
jawdatgoussous@hotmail.com
2.
Department of Architectural Engineering, Faculty of Engineering
and Technology, the University of Jordan, Amman-Jordan,
Researcher
Corresponding Author:
maryamnofal@me.com
Abstract:
This research addresses
rehabilitation and conservation of old inner-city urban center
and historic markets in Amman, Jordan. The need for urban
rehabilitation and adaptive re-use is discussed to develop and
to regenerate the urban centers, a delineation of the concept is
provided, and social aspects of rehabilitation are discussed.
The research concludes the need for Souk al-Soukar
rehabilitation and revitalization which maintain the typical
urban tissue and essential qualities of the historic site and
the life of communities working there. Moreover, it can adapt
the physical structures and organize the activities to some of
the recent requirements. In terms of urban center, it is
important to be seen as part of conservation areas, and that
their sustainability and revitalization will be most feasible if
they are integrated into new rehabilitation program.
[Jawdat Goussous, Maryam Nofal.
The Impact of Rehabilitation through Urban Regeneration on
cultural dimensions for the old markets: the case of Souk Al
Sokar, Amman, Jordan.
J Am Sci
2016;12(5):93-97].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
11.
doi:10.7537/marsjas120516.11.
Keywords:
Rehabilitation, Urban regeneration, Historic value, local
Community, open market, urban fabric |
Full Text |
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12
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The Effect of Practicing Pelvic
Rocking Exercise on Lowering Disability Level through Decreasing
Pregnancy Related Lower
Back Pain
Sahar. A. Elkheshen 1
, Hend S. Mohamed 2, Hala. A. Abdelgawad 3
1lecturer,
Nursing Department, Faculty of applied Medical Sciences, MUST
University. Giza, Egypt.
2Assistant
professor, Obstetrics and Gynecology Nursing, Faculty of Nursing
Medicine, Zagazig University,
5
Assistant professor., Faculty of physiotherapy. MUST,
University.
saharelkheshen@yahoo.com
Abstract:
Over 50 % of pregnant women experience low back pain during
their pregnancy. This pain can be intolerable, preventing them
from working, taking care of their home and children. Aim
of the study: This study aimed at assessing the effect
of practicing pelvic rocking exercise on lowering disability
level through decreasing lower back pain in late pregnancy. The
study sample comprised 100 pregnant women chosen
according to the inclusion criteria. They were divided into two
groups (50 study groups &50 control groups). Research
design: Quasi experimental design. Two tools
were used to collect data assessment sheet and the Oswestry
disability index questionnaire. Results showed that there
was a direct relationship between the period of making
exercises and their effects on decreasing disability level
regarding minimal disability level (34%, 50% & 62% after first,
second and third meetings respectively) and reverse relationship
regarding severe disability level ( 26%, 10% & 10 after first,
second and third meetings respectively). Conclusion:
Conclusion: Pelvic rocking exercise in management of lower back
pain has an important value in decreasing disability level
during pregnancy which worries the pregnant women.
[Sahar. A. Elkheshen
and Hend S. Mohamed.
The Effect of
Practicing Pelvic Rocking Exercise on Lowering Disability Level
through Decreasing Pregnancy Related Lower
Back Pain.
J Am Sci
2016;12(5):98-103].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
12.
doi:10.7537/marsjas120516.12.
Keywords:
Pelvic Rocking - Exercise –Pain – Disability Level – Nursing
Role- Physiotherapy |
Full Text |
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13
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Pigmented nevus expressed
histologically as typical basal cell carcinoma
EL-Bardaie AM and Ahmed MM
Department of Oral Pathology, Faculty of Dental Medicine, Al-Azhar
University, Cairo, Boy, Egypt
Mohabdelrzk72@yahoo.com
Abstract: Background and purpose:
It is the purpose of this article to report the first unusual
case showing no clinico-pathological correlation. A 53-year old
Caucasian man sought treatment with a chief complaint of an
itching and a slight bleeding from a common mole on the skin of
left side of the nasal bridge, started two months before seeking
medical examination. Methods: The tumor posed a
diagnostic problem by light microscopy, carful search for many
serial sections are needed. Result: The tumor cells had
features characteristic of basal cell carcinoma. Conclusion:
basal cell carcinoma, even if unusual, should be considered
in the differential diagnosis of pigmented skin lesions.
[EL-Bardaie AM and Ahmed MM.
Pigmented nevus expressed histologically as typical basal cell
carcinoma.
J Am Sci
2016;12(5):104-106].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
13.
doi:10.7537/marsjas120516.13.
Key words:
Common mole, basal cell carcinoma |
Full Text |
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14
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Production of poly β
hydroxybuthyrate from Pseudomonas putida MAO12 isolated
from wastewater sample
Majdah Mohamed Ahmed Aburas
Biology Department, Faculty of
Science, King Abdulaziz University, Jeddah, Saudi Arabia
majdah11@gmail.com
Abstract:
The biodegradable polymers of
hydroxyl butyric acid, poly β-hydroxybutyrate (PHB) is a
biodegradable material produced mainly from bacteria and has
wide range of application. In this study, 20 bacterial isolated
was obtained on Nutrient agar medium from soil and waste water
samples collected from either date palm farm or Waste water
treatment station. Out of 20 isolates, 6 (30%) showed the
highest PHB production, 8 (40%) showed moderate production and 6
(30%) were non producer. The isolate MAO12, which was isolated
from waste water sample was the most active isolate in PHB
production. It was identified as Pseudomonas putida MAO12
using morphological and physiological characters. Maximum
production was found in Nutrient broth containing 10% glucose,
at pH 7.5, 35°C after 2 days of growth at 120 rpm. The selected
bacterium were grown in Nutrient broth containing 10% of
different carbon sources and maximum percentage of PHB was found
using glucose> fructose> sucrose> maltose> cellulose >starch.
Moreover, Whey, chitin, molasses, corn steep and starch were
used as cheap carbon sources in minimal medium containing 10%
glucose. At the end of growth period, growth and PHB production
was determined. Maximum PHB was obtained by Corn steep >
Molasses > Chitin > Whey > Starch. In conclusion, PHB can be
produced by Pseudomonas putida using different cheap
carbon sources.
[Aburas MMA. Production of
poly β hydroxybuthyrate from Pseudomonas putida MAO12
isolated from wastewater sample.
J Am Sci
2016;12(5):107-112].
ISSN 1545-1003 (print); ISSN 2375-7264
(online).
http://www.jofamericanscience.org.
14.
doi:10.7537/marsjas120516.14.
Keywords:
PHB, Degradation, Production, Bioplastic, Wastes, Bacteria,
Pseudomonas putida |
Full Text |
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The
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peer-review, starting from April 9, 2016.
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